Fox E A, Gruol D L
Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.
Neurosci Lett. 1993 Jan 4;149(1):103-7. doi: 10.1016/0304-3940(93)90358-r.
Corticotropin-releasing factor (CRF) is present in climbing fibers and their terminations on Purkinje neurons. To determine whether CRF may have a postsynaptic action in this pathway, CRF effects on the electrophysiological properties of cultured cerebellar Purkinje neurons were studied. CRF produced a dose-dependent reduction of the amplitude of the afterhyperpolarization (AHP) which follows a current-induced spike train. The effect of CRF on the AHP was specific in that CRF did not significantly alter input resistance, membrane potential, amplitude of the depolarizing-off response produced at the termination of a hyperpolarizing current pulse, or the number of depolarization-induced simple spikes. Suppression of the AHP by CRF may be one mechanism by which CRF regulates the response of Purkinje neurons to other transmitters in the climbing fiber pathway or in other inputs to Purkinje neurons.
促肾上腺皮质激素释放因子（CRF）存在于攀缘纤维及其在浦肯野神经元上的终末。为了确定CRF在该通路中是否可能具有突触后作用，研究了CRF对培养的小脑浦肯野神经元电生理特性的影响。CRF使跟随电流诱发的一串动作电位之后的超极化后电位（AHP）幅度呈剂量依赖性降低。CRF对AHP的作用具有特异性，因为CRF不会显著改变输入电阻、膜电位、超极化电流脉冲终止时产生的去极化后反应幅度或去极化诱发的简单动作电位数量。CRF对AHP的抑制可能是CRF调节浦肯野神经元对攀缘纤维通路中其他递质或浦肯野神经元其他输入的反应的一种机制。
