MK-801增加大鼠顶叶皮质内源性乙酰胆碱释放：一项使用脑微透析的研究。

MK-801 increases endogenous acetylcholine release in the rat parietal cortex: a study using brain microdialysis.

作者信息

Hasegawa M, Kinoshita H, Amano M, Hasegawa T, Kameyama T, Nabeshima T

机构信息

Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University School of Medicine, Japan.

出版信息

Neurosci Lett. 1993 Feb 5;150(1):53-6. doi: 10.1016/0304-3940(93)90106-u.

DOI:10.1016/0304-3940(93)90106-u

PMID:8469404

Abstract

Glutaminergic and cholinergic neuronal interactions were investigated by using the brain microdialysis method in freely-moving rats. Acute administration of (+)-10,11-dihydro-5-methyl-5H-dibenzo[a,d]cyclohepten-5, 10-imine hydrogen maleate (MK-801) increased dose-dependently the extracellular acetylcholine (ACh) level in the rat parietal cortex. Significant increases in the extracellular ACh level were observed at doses of 0.4 and 0.5 mg/kg of MK-801, compared with the saline-treated group. The increase of extracellular ACh level was eliminated by infusion of 10(-2) M N-methyl-D-aspartate (NMDA). These results suggest that the glutaminergic neuronal system regulates functions of the cholinergic neuronal system.

摘要

采用脑微透析法在自由活动的大鼠中研究了谷氨酰胺能和胆碱能神经元的相互作用。急性给予（+）-10,11-二氢-5-甲基-5H-二苯并[a,d]环庚烯-5,10-亚胺马来酸氢盐（MK-801）可使大鼠顶叶皮质细胞外乙酰胆碱（ACh）水平呈剂量依赖性增加。与生理盐水处理组相比，在MK-801剂量为0.4和0.5mg/kg时观察到细胞外ACh水平显著升高。通过注入10（-2）M N-甲基-D-天冬氨酸（NMDA）消除了细胞外ACh水平的升高。这些结果表明，谷氨酰胺能神经元系统调节胆碱能神经元系统的功能。