Sunaga K, Chuang D M, Ishitani R
Group on Neuropharmacology, Josai University, Saitama, Japan.
Neurosci Lett. 1993 Mar 5;151(1):45-7. doi: 10.1016/0304-3940(93)90041-i.
The neurotrophic and neurosurviving effects of 9-amino-1,2,3,4-tetrahydroacridine (THA), a putative antidementia agent, were studied in cultured granule cells using biochemical and morphological methods. The addition of 30 microM THA to cultures grown in 15 mM K(+)-containing media markedly increased cell survival and enhanced [3H]N-methylscopolamine binding to muscarinic cholinergic receptors (mAChRs). Furthermore, receptor autoradiographic studies revealed that neuronal cells were labelled over both cell bodies and fibers by the [3H]receptor ligand. These observations provide direct evidence that THA promotes the expression of mAChR binding sites in differentiating cerebellar granule cells.
使用生化和形态学方法，在培养的颗粒细胞中研究了一种假定的抗痴呆药物9-氨基-1,2,3,4-四氢吖啶（THA）的神经营养和神经存活作用。向在含15 mM K⁺的培养基中生长的培养物中添加30 μM THA，可显著提高细胞存活率，并增强[³H]N-甲基东莨菪碱与毒蕈碱胆碱能受体（mAChRs）的结合。此外，受体放射自显影研究表明，神经元细胞的胞体和纤维均被[³H]受体配体标记。这些观察结果提供了直接证据，表明THA可促进分化中的小脑颗粒细胞中mAChR结合位点的表达。
