通过疏水色谱法和亲和色谱法纯化尿苷二磷酸葡萄糖脱氢酶。
Purification of UDP-glucose dehydrogenase by hydrophobic and affinity chromatography.
作者信息
Geren C R, Olomon C M, Primrose D C, Ebner K E
出版信息
Prep Biochem. 1977;7(1):19-31. doi: 10.1080/00327487708062110.
PMID:846962
Abstract
UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. The overall yield of the highly purified enzyme was 80% and the subunit molecular weight was 52,000.
摘要
通过在正亮氨酸-琼脂糖2型、琼脂糖-己烷-AMP和UDP-己醇胺-琼脂糖凝胶上进行连续层析，从牛肝丙酮粉提取物中纯化出UDP-葡萄糖脱氢酶。高度纯化的酶的总产率为80%，亚基分子量为52,000。
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