谷胱甘肽在幼年和成年大鼠顺铂肾毒性中的作用。

Role of glutathione for cisplatin nephrotoxicity in young and adult rats.

作者信息

Appenroth D, Winnefeld K

机构信息

Institute of Pharmacology and Toxicology, Friedrich Schiller University, Jena, Germany.

出版信息

Ren Fail. 1993;15(2):135-9. doi: 10.3109/08860229309046144.

DOI:10.3109/08860229309046144

PMID:8469780

Abstract

Investigations were done in 10- and 55-day-old Wistar rats. Glutathione (GSH) level in kidney was decreased by 8 mmol buthionine sulfoximine (BSO)/100 g BW. There was no effect on the renal function and nephrotoxicity of cisplatin (0.6 mg CP/100 g BW) in adult rats. In young rats BSO treatment was followed by nephrotoxic effects. Pt concentration remained unaffected by BSO in young and adult rats. GSH concentration in kidney was increased by 100 mg acetyl-cysteine (accys)/100 g BW. CP nephrotoxicity was lower in young as well as in adult ac-cys-treated rats. Pt levels in renal tissue were significantly decreased in rats from both age groups. From our results we conclude that the beneficial effect of high GSH concentration in renal tissue on CP nephrotoxicity is the result of decreased Pt concentration in kidney.

摘要

对10日龄和55日龄的Wistar大鼠进行了研究。通过每100克体重注射8毫摩尔丁硫氨酸亚砜胺（BSO）使肾脏中的谷胱甘肽（GSH）水平降低。这对成年大鼠顺铂（每100克体重0.6毫克顺铂）的肾功能和肾毒性没有影响。在幼鼠中，BSO治疗后出现了肾毒性作用。在幼鼠和成年大鼠中，BSO对铂（Pt）浓度没有影响。每100克体重注射100毫克乙酰半胱氨酸（accys）可使肾脏中的GSH浓度升高。在幼鼠和成年接受accys治疗的大鼠中，顺铂肾毒性均较低。两个年龄组大鼠肾组织中的铂水平均显著降低。从我们的结果可以得出结论，肾组织中高浓度GSH对顺铂肾毒性的有益作用是肾脏中铂浓度降低的结果。