Colbatzky F, Hermanns W
Institute of Veterinary Pathology, University of Munich, Germany.
Vet Pathol. 1993 Mar;30(2):186-94. doi: 10.1177/030098589303000212.
The clinical, hematologic, and histologic features of acute megakaryoblastic leukemia are described for an 8-year-old female Domestic Shorthair cat, a 3-year-old female mixed-breed dog, and a 3-year-old male German Shepherd Dog. The neoplastic cells were characterized as belonging to the megakaryocytic lineage. The following techniques were used: electron microscopy; detection of antibodies against human von Willebrand factor (vWF) and human platelet glycoprotein GP IIIa using a modified avidin biotin peroxidase complex technique on formalin-fixed paraffin sections; and enzyme histochemical methods on plastic sections for alkaline phosphatase, acid phosphatase, myeloperoxidase, alpha-naphthyl acetate esterase, alpha-naphthyl butyrate esterase, naphthol AS acetate esterase, and naphthol AS-D chloroacetate esterase. In addition, benign megakaryocytic cells, platelets, and neoplastic cells were labeled with lectins that have partially been shown to bind to platelet glycoproteins of other species. In healthy cats and dogs, the megakaryocytes and platelets reacted with lectins PSA, LCA, PHA-L, and WGA. Megakaryocytes and platelets from healthy cats were also labeled by lectin PNA. The lectins PHA-L and WGA reacted with neoplastic cells from the cat and both dogs. Lectin PNA bound to neoplastic cells from the cat, and lectins PSA, LCA, and SBA bound to neoplastic cells from both dogs. For the retrospective examination of paraffin-embedded material, the detection of vWF and GP IIIa appears to be the most reliable method for the identification of megakaryocytic cells.
描述了一只8岁雌性家猫、一只3岁雌性混种犬和一只3岁雄性德国牧羊犬急性巨核细胞白血病的临床、血液学和组织学特征。肿瘤细胞被鉴定为属于巨核细胞系。使用了以下技术：电子显微镜检查；在福尔马林固定的石蜡切片上，采用改良的抗生物素蛋白-生物素过氧化物酶复合物技术检测抗人血管性血友病因子（vWF）和人血小板糖蛋白GP IIIa的抗体；在塑料切片上采用酶组织化学方法检测碱性磷酸酶、酸性磷酸酶、髓过氧化物酶、α-萘乙酸酯酶、α-萘丁酸酯酶、萘酚AS乙酸酯酶和萘酚AS-D氯乙酸酯酶。此外，用部分已证明能与其他物种血小板糖蛋白结合的凝集素标记良性巨核细胞、血小板和肿瘤细胞。在健康猫和犬中，巨核细胞和血小板与凝集素PSA、LCA、PHA-L和WGA发生反应。健康猫的巨核细胞和血小板也被凝集素PNA标记。凝集素PHA-L和WGA与猫和两只犬的肿瘤细胞发生反应。凝集素PNA与猫的肿瘤细胞结合，凝集素PSA、LCA和SBA与两只犬的肿瘤细胞结合。对于石蜡包埋材料的回顾性检查，检测vWF和GP IIIa似乎是鉴定巨核细胞最可靠的方法。
