Nishikawa H, Fryer P R, Sarathchandra P, Manek S, Charlett A, Green C J
Department of Plastic Surgical Research, Mount Vernon Hospital, Northwood, Middlesex, UK.
Int J Exp Pathol. 1993 Feb;74(1):45-53.
Electron microscopy (EM) was used to evaluate ultrastructural changes in adipomusculocutaneous rat flaps which had been stored for 0, 2, 4, 6 and 8 hours under normothermic conditions (37 degrees C). The effects of treatment with desferrioxamine (DFX) or hypertonic citrate flush (HCA), prior to 4 hours of storage, were compared to untreated flaps which had been stored for 4 hours. Ultrastructural changes caused by 30 minutes of reperfusion, were also studied. Most ultrastructural alterations occurred between 2 and 4 hours of warm storage and there were further changes in some cells after short periods of reperfusion. DFX decreased smooth muscle damage and HCA protected adipocytes but neither of these agents preserved endothelial cells. These studies indicated that free radical-dependent and independent mechanisms were both involved in events which led to flap necrosis after periods of warm storage and reperfusion.
采用电子显微镜（EM）评估在常温条件（37摄氏度）下储存0、2、4、6和8小时的大鼠脂肪肌皮瓣的超微结构变化。将去铁胺（DFX）或高渗柠檬酸盐冲洗液（HCA）在储存4小时前进行处理的效果与未处理且已储存4小时的皮瓣进行比较。还研究了30分钟再灌注引起的超微结构变化。大多数超微结构改变发生在温暖储存的2至4小时之间，并且在短时间再灌注后一些细胞出现了进一步变化。DFX减少了平滑肌损伤，HCA保护了脂肪细胞，但这两种药物均未保护内皮细胞。这些研究表明，自由基依赖性和非依赖性机制均参与了导致温暖储存和再灌注后皮瓣坏死的事件。
