Acland R D, Anderson G, Siemionow M, McCabe S
Department of Surgery, University of Louisville, Ky.
Plast Reconstr Surg. 1989 Aug;84(2):280-8; discussion 289.
This study was done to determine whether microemboli are produced by an arterial anastomosis. Direct in vivo observations were made in an isolated microcirculatory bed lying directly downstream from a newly made anastomosis. The tissue used was the isolated rat cremaster muscle, a new experimental model. The vessel anastomosed was the external iliac artery. Following anastomosis, microemboli were clearly observed in eight of eight animals during the first 30 minutes after clamp release. Embolic events were sometimes of impressive magnitude and in one case were associated with cessation of blood flow throughout the preparation. No microemboli were observed in eight of eight animals subjected only to dissection of the cremaster, nor were any observed in eight of eight animals in which the isolated cremaster was subjected only to 2 hours of clamp ischemia. These findings may be significant in explaining perturbations to blood flow following free-tissue transfer and instances of partial tissue necrosis following apparently successful arterial repair. These findings also identify an important factor (microemboli) to be considered in research on reperfusion injury.
本研究旨在确定动脉吻合术是否会产生微栓子。在新制作的吻合口下游直接的孤立微循环床中进行了直接的体内观察。所使用的组织是孤立的大鼠提睾肌，这是一种新的实验模型。吻合的血管是髂外动脉。吻合后，在松开血管夹后的最初30分钟内，8只动物中有8只清晰地观察到了微栓子。栓塞事件有时规模惊人，在一个案例中与整个标本的血流停止有关。仅对提睾肌进行解剖的8只动物中，没有观察到微栓子；仅对孤立提睾肌进行2小时血管夹缺血处理的8只动物中，也没有观察到微栓子。这些发现对于解释游离组织移植后血流的扰动以及明显成功的动脉修复后部分组织坏死的情况可能具有重要意义。这些发现还确定了在再灌注损伤研究中需要考虑的一个重要因素（微栓子）。
