Dunn A L, Crnic L S
Department of Psychiatry, University of Colorado Health Sciences Center, Denver 80262.
Brain Behav Immun. 1993 Mar;7(1):104-11. doi: 10.1006/brbi.1993.1011.
Interferon-alpha decreases food intake and activity in mice and humans. The present study further explores parallels between effects of this protein in humans and mice, using a hybrid recombinant interferon-alpha A/D (INF-alpha A/D) that has antiviral and hepatic activity in mice. Measures of activity (open field), muscle strength (forelimb grip strength), and motor ability (swim posture and endurance with 0, 3, and 6% added weight) were examined in Balb/c mice injected with 1600 U/g of hybrid rhuIFN-alpha A/D daily for 5 days (n = 9) or with IFN vehicle (n = 11). Open field activity was significantly depressed in the group exposed to IFN. The number of times the nose dipped under the water when swimming with 3% added body weight was higher in the IFN-treated mice, while float time was decreased with 6% added weight. The IFN-treated mice were slower to adopt the new strategies necessary to swim with added weight. Depression of motor activity is a robust, general effect of IFN treatment observed in this as well as previously published studies. Together, these studies demonstrate activity decrements in two different strains of mice, two different activity measures, acute and chronic injections, and recombinant and nonrecombinant IFN preparations.
干扰素-α可减少小鼠和人类的食物摄入量及活动量。本研究使用在小鼠中具有抗病毒和肝脏活性的杂交重组干扰素-α A/D（INF-α A/D），进一步探究了这种蛋白质在人类和小鼠中的作用异同。对每天注射1600 U/g杂交重组人干扰素-α A/D共5天的Balb/c小鼠（n = 9）或注射干扰素赋形剂的小鼠（n = 11），检测其活动量（旷场实验）、肌肉力量（前肢握力）和运动能力（游泳姿势以及在添加0%、3%和6%体重负荷时的耐力）。接受干扰素处理的组旷场活动量显著降低。在添加3%体重负荷游泳时，接受干扰素处理的小鼠鼻子浸入水中的次数更多，而在添加6%体重负荷时漂浮时间减少。接受干扰素处理的小鼠在带着增加的体重游泳时，采用新策略的速度较慢。运动活动量降低是在本研究以及先前发表的研究中观察到的干扰素治疗的一个显著的普遍效应。总之，这些研究表明，在两种不同品系的小鼠、两种不同的活动量测量方法、急性和慢性注射以及重组和非重组干扰素制剂中，活动量均有下降。
