亲水性自旋捕获剂5,5-二甲基-1-吡咯啉-1-氧化物不能保护大鼠心脏免受再灌注损伤。

The hydrophilic spin trap, 5,5-dimethyl-1-pyrroline-1-oxide, does not protect the rat heart from reperfusion injury.

作者信息

Bradamante S, Jotti A, Paracchini L, Monti E, Morti E [corrected to Monti E ]

机构信息

CNR, Centro Sintesi e Stereochimica Speciali Sistemi Organici, Milano, Italy.

出版信息

Eur J Pharmacol. 1993 Mar 30;234(1):113-6. doi: 10.1016/0014-2999(93)90713-r.

DOI:10.1016/0014-2999(93)90713-r

PMID:8472754

Abstract

The role and site of free radical generation in myocardial ischemia/reperfusion injury were investigated using the hydrophilic spin trapping agent, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). DMPO (40 mM) proved ineffective in preserving the contractile performance and energy metabolism of Langendorff-perfused rat hearts following ischemia and reperfusion. This result, which is in contrast with the cardioprotection observed with hydrophobic spin traps, suggests that free radicals are generated intracellularly under these conditions.

摘要

使用亲水性自旋捕获剂5,5 - 二甲基 - 1 - 吡咯啉 - 1 - 氧化物（DMPO）研究了自由基在心肌缺血/再灌注损伤中的产生作用和部位。结果表明，DMPO（40 mM）在缺血和再灌注后对Langendorff灌注的大鼠心脏的收缩性能和能量代谢并无保护作用。这一结果与疏水性自旋捕获剂所观察到的心脏保护作用相反，提示在这些条件下自由基是在细胞内产生的。