Ballin A, Sagi O, Schiby G, Meytes D
Department of Haematology, Sackler School of Medicine, Tel Aviv, Israel.
Eur J Haematol. 1993 Mar;50(3):168-71. doi: 10.1111/j.1600-0609.1993.tb00086.x.
Recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) administered to bone marrow (BM) transplant recipients is associated with earlier recovery. We have investigated the possibility of stimulating normal donor mice in vivo with GM-CSF. Donor balb/c mice were injected i.p. with GM-CSF (5000 u) or saline. Seventy-two hours later 5 x 10(5)BM cells from either GM-CSF-treated or control donors were infused into lethally irradiated (850 R) recipients. In the recipients of BM from GM-CSF-treated donors, significantly higher CFU-S and significantly higher survival rate (57% [n = 65]; vs. 30% [n = 63]; p < 0.05) were noted. Donor mice of the GM-CSF group did not differ in bone-marrow cellularity and composition from their controls. However, recipients of BM from GM-CSF-treated mice had higher blood counts of haemoglobin, leukocytes and platelets compared to controls. These data demonstrate that pretreatment of BM donors with GM-CSF may be of benefit in improving survival and marrow engraftment in mice.
给予骨髓移植受者重组粒细胞巨噬细胞集落刺激因子（GM-CSF）与更快恢复相关。我们研究了在体内用GM-CSF刺激正常供体小鼠的可能性。给供体Balb/c小鼠腹腔注射GM-CSF（5000单位）或生理盐水。72小时后，将来自GM-CSF处理组或对照组供体的5×10⁵个骨髓细胞注入接受致死性照射（850拉德）的受体小鼠体内。在接受GM-CSF处理的供体小鼠骨髓的受体中，观察到显著更高的脾集落形成单位（CFU-S）和显著更高的存活率（57%[n = 65]；对比30%[n = 63]；p < 0.05）。GM-CSF组的供体小鼠骨髓细胞数量和组成与对照组无差异。然而，与对照组相比，接受GM-CSF处理小鼠骨髓的受体血红蛋白、白细胞和血小板的血细胞计数更高。这些数据表明，用GM-CSF预处理骨髓供体可能有利于提高小鼠的存活率和骨髓植入率。
