Ransohoff R M, Hamilton T A, Tani M, Stoler M H, Shick H E, Major J A, Estes M L, Thomas D M, Tuohy V K
Research Institute, Cleveland Clinic Foundation, Ohio 44195.
FASEB J. 1993 Apr 1;7(6):592-600. doi: 10.1096/fasebj.7.6.8472896.
Mononuclear leukocytes preferentially accumulate in the central nervous system (CNS) during the course of experimental autoimmune encephalomyelitis (EAE). To address factors that govern leukocyte trafficking in EAE, we monitored expression of mRNAs encoding IP-10 and JE/MCP-1, which are members of a family of chemoattractant cytokines. A transient burst of IP-10 and JE/MCP-1 mRNA accumulation in the CNS occurred, in close relation to the onset of histologic and clinical disease. In situ hybridizations showed, unexpectedly, that astrocytes were the major source of mRNAs encoding IP-10 and JE/MCP-1. These observations implicate astrocyte-derived cytokines as potential chemoattractants for inflammatory cells during EAE.
在实验性自身免疫性脑脊髓炎（EAE）病程中，单核白细胞优先在中枢神经系统（CNS）中积聚。为了研究调控EAE中白细胞迁移的因素，我们监测了编码IP - 10和JE/MCP - 1的mRNA的表达，它们是趋化因子细胞因子家族的成员。CNS中IP - 10和JE/MCP - 1 mRNA积累出现短暂爆发，与组织学和临床疾病的发作密切相关。原位杂交意外地显示，星形胶质细胞是编码IP - 10和JE/MCP - 1的mRNA的主要来源。这些观察结果表明，星形胶质细胞衍生的细胞因子可能是EAE期间炎症细胞的潜在趋化因子。
