Lee N A, Clark D P
Department of Microbiology, Southern Illinois, University, Carbondale 62901.
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):151-5. doi: 10.1111/j.1574-6968.1993.tb06022.x.
A natural isolate, designated BSA56, which was originally selected for growth with benzene sulfonic acid as sole carbon and energy source, was identified as a strain of Pseudomonas maltophila. Strain BSA56 grew on a wide range of aromatic sulfonic acids and was shown to release sulfite from benzene sulfonic acid and 2-naphthalene sulfonic acid. Although it also grew on toluene sulfonic acid and pyridine sulfonic acid, no significant sulfite release was observed with these substrates. Release of sulfite from benzene sulfonic acid was greatly promoted by the presence of glycerol. The ability to release sulfite was induced by growth in the presence of benzene sulfonic acid and was repressed almost entirely by substrates allowing rapid growth such as acetate. Strain BSA56 grew better at 30 degrees C than 37 degrees C on most aromatic substrates, but the reverse was true for most aromatic sulfonates. Several mutants of BSA56 were isolated with defects in benzoate, salicylate, or gentisate metabolism. However, all these mutants retained the ability to degrade the aromatic sulfonates.
一种天然分离株，命名为BSA56，最初是因其能以苯磺酸作为唯一碳源和能源进行生长而被筛选出来的，它被鉴定为嗜麦芽窄食单胞菌菌株。BSA56菌株能在多种芳香族磺酸上生长，并被证明能从苯磺酸和2-萘磺酸中释放亚硫酸盐。尽管它也能在甲苯磺酸和吡啶磺酸上生长，但在这些底物上未观察到明显的亚硫酸盐释放。甘油的存在极大地促进了苯磺酸中亚硫酸盐的释放。释放亚硫酸盐的能力是在苯磺酸存在下生长时诱导产生的，而几乎完全被允许快速生长的底物（如乙酸盐）所抑制。在大多数芳香族底物上，BSA56菌株在30℃时比在37℃时生长得更好，但对于大多数芳香族磺酸盐来说情况则相反。分离出了几株BSA56的突变体，它们在苯甲酸、水杨酸盐或龙胆酸盐代谢方面存在缺陷。然而，所有这些突变体都保留了降解芳香族磺酸盐的能力。
