Cahill E S, O'Hagan D T, Illum L, Redhead K
Department of Pharmaceutical Sciences, Nottingham University, UK.
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):211-6. doi: 10.1111/j.1574-6968.1993.tb06032.x.
Intra-nasal immunization of mice with purified Bordetella pertussis filamentous haemagglutinin (FHA) or a crude cell sonicate was shown to protect against subsequent B. pertussis aerosol challenge. Immunization with FHA was found to be the most effective and resulted in complete clearance of the bacterial infection from the lungs within 14 days. Serum IgG and lung IgA anti-FHA antibodies were detectable within 4 weeks of the first immunization and anamnestic responses were seen following secondary immunization and subsequent challenge with B. pertussis. Nasal administration of pertussis antigens is a route which induces good systemic serum, as well as local secretory, antibody responses.
用纯化的百日咳博德特氏菌丝状血凝素（FHA）或粗制细胞超声裂解物对小鼠进行鼻内免疫，可使其免受随后的百日咳博德特氏菌气溶胶攻击。研究发现，用FHA免疫最为有效，能在14天内使肺部的细菌感染完全清除。首次免疫后4周内可检测到血清IgG和肺部IgA抗FHA抗体，二次免疫及随后用百日咳博德特氏菌攻击后可观察到回忆反应。经鼻给予百日咳抗原是一种既能诱导良好的全身性血清抗体反应，又能诱导局部分泌性抗体反应的途径。
