Barja F, Chappuis M L, Turian G
Laboratory of General Microbiology, University of Geneva, Switzerland.
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):261-6. doi: 10.1111/j.1574-6968.1993.tb06040.x.
Anti-actin drugs, cytochalasins A and B, inhibited both normal single, and benomyl-induced multiple, germ tube outgrowth from conidia of Neurospora crassa. Actin was cytochemically found to be concentrated in each of the benomyl-induced germ tube tips. No significant quantitative changes either in total actin or its isoforms were measured in the inhibitor-treated germlings. While intact microtubules are required for normal, monopolar axiation of the germ tube, they appear not to be necessary for benomyl-induced multipolar outgrowth which, in contrast, still requires intact actin microfilaments. Microfilaments and microtubules thus play complementary roles in the normal germination of conidia.
抗肌动蛋白药物细胞松弛素A和B，抑制了粗糙脉孢菌分生孢子正常的单生芽管以及苯菌灵诱导的多生芽管的生长。通过细胞化学方法发现肌动蛋白集中在苯菌灵诱导的每个芽管顶端。在抑制剂处理的菌丝体中，未检测到总肌动蛋白或其同工型有显著的定量变化。虽然完整的微管对于芽管正常的单极轴形成是必需的，但它们对于苯菌灵诱导的多极生长似乎不是必需的，相反，这种多极生长仍然需要完整的肌动蛋白微丝。因此，微丝和微管在分生孢子的正常萌发中发挥互补作用。
