利用与哈维氏弧菌MAV的luxAB融合来定量肉葡萄球菌TM300中木糖操纵子的诱导和分解代谢物阻遏。
Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300.
作者信息
Sizemore C, Geissdörfer W, Hillen W
机构信息
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie und Biochemie der Friedrich-Alexander Universität Erlangen-Nürnberg, FRG.
出版信息
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):303-6. doi: 10.1111/j.1574-6968.1993.tb06047.x.
The luxA,B genes from the Gram-negative marine bacterium Vibrio harveyi MAV were used in Staphylococcus carnosus TM300 as a reporter system for regulated expression of xylose utilization. The luciferase genes were fused to the xyl operon from Staphylococcus xylosus C2a. Expression of bioluminescence was induced through addition of xylose and repressed in the presence of glucose. A method to quantitate bioluminescence directly from the culture is described.
革兰氏阴性海洋细菌哈维氏弧菌MAV的luxA、B基因被用于肉葡萄球菌TM300中，作为木糖利用调控表达的报告系统。荧光素酶基因与木糖葡萄球菌C2a的木糖操纵子融合。通过添加木糖诱导生物发光表达，在葡萄糖存在时则受到抑制。本文描述了一种直接从培养物中定量生物发光的方法。
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