Arhin F F, Vining L C
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Gene. 1993 Apr 15;126(1):129-33. doi: 10.1016/0378-1119(93)90601-x.
Genes involved in the biosynthesis of p-aminobenzoic acid (PABA) in Streptomyces lividans 1326 were cloned in pBR322 by complementing a pabB mutant of Escherichia coli. A 2.7-kb BamHI-SstI fragment of the cloned DNA complemented pabA and pabB mutations in both E. coli and S. lividans; complementation in S. lividans was accompanied by integration of the recombinant plasmid into the host chromosome. The nucleotide (nt) sequence of the 2.7-kb fragment contained two open reading frames, the deduced amino acid sequences of which were similar to those of pabA and pabB products from other bacteria. The nt sequences indicated that pabA and pabB are closely linked in S. lividans and supported cloning evidence that the genes are expressed from a promoter with features resembling those of most E. coli promoters.
通过对大肠杆菌的对氨基苯甲酸（PABA）生物合成相关基因的pabB突变体进行互补，将参与天蓝色链霉菌1326中PABA生物合成的基因克隆到pBR322中。克隆DNA的一个2.7 kb BamHI-SstI片段可互补大肠杆菌和天蓝色链霉菌中的pabA和pabB突变；在天蓝色链霉菌中的互补伴随着重组质粒整合到宿主染色体中。2.7 kb片段的核苷酸（nt）序列包含两个开放阅读框，其推导的氨基酸序列与其他细菌的pabA和pabB产物的序列相似。nt序列表明，pabA和pabB在天蓝色链霉菌中紧密相连，并支持克隆证据，即这些基因由一个具有与大多数大肠杆菌启动子相似特征的启动子表达。
