鱼发光杆菌中鲁比萤光素蛋白编码基因与lux操纵子相连。
The lumazine protein-encoding gene in Photobacterium leiognathi is linked to the lux operon.
作者信息
Lin J W, Chao Y F, Weng S F
机构信息
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, ROC.
出版信息
Gene. 1993 Apr 15;126(1):153-4. doi: 10.1016/0378-1119(93)90605-3.
The nucleotide (nt) sequence of the lumP (EMBL accession No. X65612) gene of Photobacterium leiognathi PL741 was determined and the amino acid (aa) sequence deduced. The encoded aa sequence of lumP was identified as that of the lumazine protein (LumP) by homology with that of Photobacterium phosphoreum (56%). This small protein has a calculated M(r) of 19,997 and comprises 186 aa residues. Biochemical studies suggested that LumP is the protein which, when combined with luciferase, is responsible for the bioluminescent spectrum shift from blue-green light (490-505 nm) to blue (470 nm) in P. leiognathi. The nt sequence of the flanking region showed that lumP is linked to the lux operon but runs in the opposite direction. The gene order of the lumP and lux operon is as follows: <--lumP-R&R-luxC-luxD-luxA-luxB-luxN-lu xE-->; the R&R regulatory region sequence included two promoter systems, PR for the lux operon and PL for the lumP or the lum operon.
测定了鱼发光杆菌PL741的lumP基因（EMBL登录号：X65612）的核苷酸（nt）序列，并推导了其氨基酸（aa）序列。通过与磷光杆菌的氨基酸序列进行同源性比较（56%），确定lumP编码的氨基酸序列为核黄素蛋白（LumP）的序列。这种小蛋白的计算分子量（M(r)）为19,997，由186个氨基酸残基组成。生化研究表明，LumP是一种蛋白质，当与荧光素酶结合时，它负责鱼发光杆菌的生物发光光谱从蓝绿光（490 - 505 nm）转变为蓝光（470 nm）。侧翼区域的核苷酸序列表明，lumP与lux操纵子相连，但转录方向相反。lumP和lux操纵子的基因顺序如下：<--lumP-R&R-luxC-luxD-luxA-luxB-luxN-lu xE-->；R&R调控区域序列包括两个启动子系统，lux操纵子的PR和lumP或lum操纵子的PL。
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