Woodward B J, Lenton E A, Mac Neil S
Sheffield Fertility Centre, UK.
Hum Reprod. 1993 Feb;8(2):272-6. doi: 10.1093/oxfordjournals.humrep.a138036.
The objective of this study was to investigate the requirement for calmodulin in the cell division of the early human preimplantation embryo. Experiments using three agents capable of inhibiting calmodulin activity, N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide (W7), W7 linked to agarose beads (W7-agarose) and calmodulin antibody, showed that W7 and calmodulin antibody arrested division of embryos in a dose-dependent manner. As W7 is able to penetrate the zona pellucida and enter the cells, calmodulin antibody can traverse the zona but not enter the cells and W7-agarose cannot traverse the zona, we have deduced that the calmodulin which appears relevant to embryo division may be both intracellular and intrazonal but not extrazonal. We conclude that calmodulin is specifically required for cell division in the early human preimplantation embryo and that the concentration of calmodulin surrounding the embryo within the zona is particularly important to embryo development.
本研究的目的是调查钙调蛋白在人类早期植入前胚胎细胞分裂中的需求。使用三种能够抑制钙调蛋白活性的试剂进行的实验，即N-(6-氨基己基)-5-氯-1-萘磺酰胺（W7）、与琼脂糖珠相连的W7（W7-琼脂糖）和钙调蛋白抗体，表明W7和钙调蛋白抗体以剂量依赖的方式阻止胚胎分裂。由于W7能够穿透透明带并进入细胞，钙调蛋白抗体可以穿过透明带但不进入细胞，而W7-琼脂糖不能穿过透明带，我们推断与胚胎分裂相关的钙调蛋白可能既存在于细胞内也存在于透明带内，但不存在于透明带外。我们得出结论，钙调蛋白是人类早期植入前胚胎细胞分裂所特需的，并且透明带内胚胎周围的钙调蛋白浓度对胚胎发育尤为重要。
