利用合成的Eco RI位点构建的海胆DNA单个重复序列的克隆。
Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites.
作者信息
Scheller R H, Thomas T L, Lee A S, Klein W H, Niles W D, Britten R J, Davidson E H
出版信息
Science. 1977 Apr 8;196(4286):197-200. doi: 10.1126/science.847467.
PMID:847467
Abstract
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.
摘要
通过复性至低Cot值，然后用核酸酶SI处理，从海胆DNA中分离出散布重复序列。将合成的Eco RI位点连接到重复序列元件上，然后将其插入质粒RSF2124的Eco RI位点并进行克隆。可以用Eco RI从质粒中切下重复序列用于进一步研究。
Zotero 插件