Scheller R H, Thomas T L, Lee A S, Klein W H, Niles W D, Britten R J, Davidson E H
Science. 1977 Apr 8;196(4286):197-200. doi: 10.1126/science.847467.
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.
通过复性至低Cot值，然后用核酸酶SI处理，从海胆DNA中分离出散布重复序列。将合成的Eco RI位点连接到重复序列元件上，然后将其插入质粒RSF2124的Eco RI位点并进行克隆。可以用Eco RI从质粒中切下重复序列用于进一步研究。
