Val'kiunas G A, Ezhova T A
Parazitologiia. 1993 Jan-Feb;27(1):19-35.
Observations were made through examination of thin blood films which were prepared at appropriate intervals from citrated fresh blood of infected birds. The comparative description of gametogenesis, zygote and ookinete formation of Haemoproteus tartakovskyi, H. belopolskyi, H. fringillae, H. pallidus under the light microscope is given for the first time. This process has a general pattern for all investigated haemoproteid species. It is shown that macrogametocyte has been transformed directly to macrogamete without forming a residual body, as it was noted by Desser (1972) for H. (= Parahaemoproteus) velans. The most informative signs, which can be used for haemoproteid systematics, were determined. Among them microgamete length, zygote structure, morphological features of developing ookinete as well as the rate of ookinete formation can be pointed out first of all. H. pallidus microgametes are twice shorter than H. tartakovskyi, H. belopolskyi, H. fringillae ones. A large clear vacuole is formed in zygote of H. fringillae only. The majority of diagnostic features has the developing ookinetes. On the basis of ookinetes morphology the investigated species of haemoproteids can be determined easily. There were clear differences between haemoproteid species on the rate of ookinete formation. The ookinetes of H. belopolskyi and H. fringillae are formed for about 12 times more slowly than H. pallidus ones, and for about 6 times more rapidly than H. tartakovskyi ones. The peculiarities of gametogenesis, zygote and ookinete formation under the stable conditions in vitro can be used as usefull additional signs for determination of haemoproteid species.
通过检查薄血膜进行观察，薄血膜是从感染鸟类的枸橼酸盐新鲜血液中在适当间隔制备的。首次在光学显微镜下对塔氏血变原虫、别氏血变原虫、燕血变原虫、苍血变原虫的配子发生、合子和动合子形成进行了比较描述。这一过程在所有研究的血变原虫物种中具有普遍模式。结果表明，大配子母细胞直接转化为大配子，未形成残留体，正如德塞尔（1972年）对韦氏血变原虫（=副血变原虫）所指出的那样。确定了可用于血变原虫分类学的最具信息性的特征。其中首先可以指出小配子长度、合子结构、发育中动合子的形态特征以及动合子形成速率。苍血变原虫的小配子比塔氏血变原虫、别氏血变原虫、燕血变原虫的小配子短两倍。仅在燕血变原虫的合子中形成一个大的透明液泡。大多数诊断特征存在于发育中的动合子中。根据动合子形态可以很容易地确定所研究的血变原虫物种。血变原虫物种在动合子形成速率上存在明显差异。别氏血变原虫和燕血变原虫的动合子形成速度比苍血变原虫慢约12倍，比塔氏血变原虫快约6倍。体外稳定条件下配子发生、合子和动合子形成的特点可作为确定血变原虫物种的有用附加特征。
