抗体介导的对致死性脑内新型隐球菌感染小鼠的保护作用。
Antibody-mediated protection in mice with lethal intracerebral Cryptococcus neoformans infection.
作者信息
Mukherjee J, Pirofski L A, Scharff M D, Casadevall A
机构信息
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3636-40. doi: 10.1073/pnas.90.8.3636.
The fungus Cryptococcus neoformans is an important opportunistic pathogen for patients with AIDS. C. neoformans infections frequently involve the brain and are often fatal. In the setting of AIDS C. neoformans infections are incurable and new treatment strategies are urgently needed. Passive administration of antibody is a potential therapeutic option for the prevention and treatment of C. neoformans. The IgG1 murine monoclonal antibody 2H1 to the capsular polysaccharide of C. neoformans was studied for its ability to modify the course of lethal intracerebral cryptococcal infection in mice. Intraperitoneal administration of antibody 2H1 resulted in small, yet significant, prolongations in the average survival of mice given intracerebral infection and reduced the number of C. neoformans colonies in brain tissue. Histopathological examination of brain tissues revealed a diffuse cryptococcal meningitis with fewer organisms in the brains of mice that received antibody 2H1 than in the control group. Thus, systemic administration of a monoclonal antibody can modify the course of lethal intracerebral C. neoformans infection in mice by prolonging survival and decreasing fungal burden in brain tissues.
新型隐球菌是艾滋病患者重要的机会性致病菌。新型隐球菌感染常累及脑部，且往往致命。在艾滋病背景下，新型隐球菌感染无法治愈，迫切需要新的治疗策略。被动给予抗体是预防和治疗新型隐球菌的一种潜在治疗选择。研究了针对新型隐球菌荚膜多糖的IgG1鼠单克隆抗体2H1改变小鼠致死性脑内隐球菌感染病程的能力。腹腔注射抗体2H1可使脑内感染小鼠的平均存活时间略有延长，但具有显著意义，同时减少了脑组织中新型隐球菌菌落的数量。脑组织的组织病理学检查显示，与对照组相比，接受抗体2H1的小鼠脑部为弥漫性隐球菌脑膜炎，菌体量较少。因此，全身给予单克隆抗体可通过延长存活时间和减少脑组织中的真菌负荷来改变小鼠致死性脑内新型隐球菌感染的病程。
Zotero 插件