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用苯乙醛处理的细菌的光子发射。革兰氏阳性菌和革兰氏阴性菌之间可能的区别。

Photon emission by bacteria challenged with phenylacetaldehyde. A possible distinction between gram-positive and gram-negative bacteria.

作者信息

Surpili M J, Faljoni-Alario A, Cilento G

机构信息

Department of Biochemistry, Instituto de Química, Universidade de Sao Paulo.

出版信息

Photochem Photobiol. 1993 Mar;57(3):564-9. doi: 10.1111/j.1751-1097.1993.tb02334.x.

DOI:10.1111/j.1751-1097.1993.tb02334.x
PMID:8475191
Abstract

With all bacteria tested, addition of phenylacetaldehyde leads to light emission. The latter is markedly stronger with gram-negative bacteria, presumably because they possess a thinner wall and an extra external lipophilic membrane. Consistent with this explanation, the bactericidal effect of phenylacetaldehyde is also stronger with gram-negative bacteria. The spectrum of the emitted light shows maximal emission in the 500 nm region and is very similar to that observed when a protein (bovine serum albumin), free amino acids or isopropylamine reacts with phenylacetaldehyde.

摘要

对所有测试的细菌而言,添加苯乙醛会导致发光。革兰氏阴性菌的发光明显更强,推测是因为它们的细胞壁较薄且有一层额外的外部亲脂性膜。与这一解释相符的是,苯乙醛对革兰氏阴性菌的杀菌作用也更强。发射光的光谱在500纳米区域显示出最大发射,并且与蛋白质(牛血清白蛋白)、游离氨基酸或异丙胺与苯乙醛反应时观察到的光谱非常相似。

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