Crespo P V, Lopez-Escamez J A, Cañizares F J, Campos A
Department of Histology and Cellular Biology, Faculty of Medicine, University of Granada, Spain.
Acta Otolaryngol. 1993 Mar;113(2):176-80. doi: 10.3109/00016489309135788.
Electron probe X-ray microanalysis was used to determine the concentrations of P, S and K (Cp, CS, CK) in the gelatinous membrane of the mouse utricle. The otolithic membranes were plunge-frozen in liquid N2, freeze-dried and carbon-coated. Quantitative analysis was carried out with an energy dispersive detector using the peak-to-background ratio method and different concentrations of KH2PO4 and K2SO4 salts dissolved in dextran solutions to calibrate the microprobe. P, S and K were measured and their concentrations plotted as bar graphs to study the frequency distributions. Regression analysis revealed a dependence between the concentrations of P and K (CK = 1454.10 - 2.83 CP, r = -0.68745, p < 0.05), and P and S (CS = 43.18 + 0.23 CP, r = 0.66949, p < 0.05); however, no correlation was found between CK and CP (r = -0.25424). The findings obtained in the present study show an inverse relationship between P and K ions, and direct relationship between P and S in the gelatinous membrane of the utricle.
采用电子探针X射线微分析法测定小鼠椭圆囊凝胶膜中磷（P）、硫（S）和钾（K）的浓度（Cp、CS、CK）。耳石膜在液氮中骤冷、冻干并进行碳涂层处理。使用能量色散探测器，采用峰背比法，并用溶解在葡聚糖溶液中的不同浓度的KH2PO4和K2SO4盐对微探针进行校准，进行定量分析。测量P、S和K，并将其浓度绘制成柱状图以研究频率分布。回归分析显示P和K的浓度之间存在相关性（CK = 1454.10 - 2.83 CP，r = -0.68745，p < 0.05），以及P和S之间存在相关性（CS = 43.18 + 0.23 CP，r = 0.66949，p < 0.05）；然而，未发现CK和CP之间存在相关性（r = -0.25424）。本研究的结果表明，椭圆囊凝胶膜中P和K离子之间呈负相关，P和S之间呈正相关。
