Roberto M, Favia A, Lozupone E
Department of Bioacoustics, University of Bari, Italy.
Acta Otolaryngol. 1993 Mar;113(2):181-6. doi: 10.3109/00016489309135789.
The incus of the right ear from 4 growing mongrel dogs was surgically disarticulated and left in the middle ear space. The external auditory canal was then filled with teflon paste and sutured. After 6 weeks (D-6 group) and 13 weeks (D-13 group) the animals were sacrificed. The right experimental incus and the left control one were embedded in methyl methacrylate and sectioned in single 50-microns-thick sections according to the principal axis of the two processes. On the microradiographs of each section we evaluated the thickness of the body and of both processes and the percentage area of the primary channels of the secondary osteons and that of the appositional bone tissue. The thickness of the body and of the two processes was more pronounced in all the experimental incuses, in which 6% (in D-6) and 8% (in D-13) of the total area were occupied by new appositional woven bone. In the body of the D-13 group, 9% of the pre-existing bone was substituted by secondary osteons. The results indicate that the incus react to the variations of mechanical stimuli.
对4只生长中的杂种犬的右耳砧骨进行手术离断，并留在中耳腔。然后用聚四氟乙烯糊剂填充外耳道并缝合。6周后（D-6组）和13周后（D-13组）处死动物。将右侧实验性砧骨和左侧对照砧骨嵌入甲基丙烯酸甲酯中，并根据两个突起的主轴切成50微米厚的单切片。在每个切片的显微放射照片上，我们评估了砧骨体和两个突起的厚度、二级骨单位初级通道的面积百分比以及附加骨组织的面积百分比。在所有实验性砧骨中，砧骨体和两个突起的厚度都更明显，其中新的附加编织骨分别占总面积的6%（D-6组）和8%（D-13组）。在D-13组的砧骨体中，9%的原有骨被二级骨单位替代。结果表明，砧骨对机械刺激的变化有反应。
