Crestani B, Rolland C, Petiet A, Colas-Linhart N, Aubier M
Institut National de la Santé et de la Recherche Médicale U. 226, Paris, France.
Am J Physiol. 1993 Apr;264(4 Pt 1):L391-400. doi: 10.1152/ajplung.1993.264.4.L391.
We characterized the influence of phosphorylated sugars and cell surface sialic acids on the adherence of human polymorphonuclear leukocyte (PMN) to rat alveolar type II cell (ATII cells) and human-derived A549 cell monolayers in vitro. Percent adherence of radiolabeled polymorphonuclear leukocytes was assessed after incubating cells with the carbohydrates, enzymes, or lectins to be tested. Lactose-1-phosphate (Lact1P) and maltose-1-phosphate (Malt1P) (10 mM) inhibited adherence of PMN to ATII cells and A549 cells. Maximal inhibition followed treatment of both PMN and rat ATII cells and amounted to 85 +/- 7% with Lact1P and 92 +/- 3% with Malt1P. Inhibition was concentration dependent. Incubation of PMN with mannose-6-phosphate reduced adherence to rat ATII cells and A549 cells by 36 +/- 11 and 39 +/- 8%, respectively. Maximal concentrations of sugars did not alter cellular viability. Neuraminidase-induced desialilation of ATII cells increased adherence of PMN by 36 +/- 7% to rat ATII cells and by 86 +/- 18% to A549 cells. Masking of terminal sialic acids on rat ATH cells with Limulus polyphemus agglutinin (100 micrograms/ml) increased adherence by 50 +/- 2%. These results indicate that cell surface carbohydrates are involved in the regulation of the adhesive interaction between PMN and ATII cells in vitro.
我们在体外研究了磷酸化糖和细胞表面唾液酸对人多形核白细胞（PMN）与大鼠II型肺泡细胞（ATII细胞）及人源A549细胞单层黏附的影响。在用待测的碳水化合物、酶或凝集素孵育细胞后，评估放射性标记的多形核白细胞的黏附百分比。1 - 磷酸乳糖（Lact1P）和1 - 磷酸麦芽糖（Malt1P）（10 mM）可抑制PMN与ATII细胞及A549细胞的黏附。对PMN和大鼠ATII细胞进行处理后，抑制作用达到最大值，Lact1P的抑制率为85±7%，Malt1P为92±3%。抑制作用呈浓度依赖性。用6 - 磷酸甘露糖孵育PMN后，其与大鼠ATII细胞及A549细胞的黏附分别减少36±11%和39±8%。糖的最大浓度不会改变细胞活力。神经氨酸酶诱导的ATII细胞去唾液酸化使PMN与大鼠ATII细胞的黏附增加36±7%，与A549细胞的黏附增加86±18%。用鲎试剂凝集素（100微克/毫升）封闭大鼠ATII细胞上的末端唾液酸，可使黏附增加50±2%。这些结果表明，细胞表面碳水化合物参与了体外PMN与ATII细胞间黏附相互作用的调节。
