Valentinis B, Zaffaroni N, Sturani E, Martegani E, Silvestrini R
Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
Anticancer Res. 1993 Jan-Feb;13(1):125-8.
The reliability of the competitive polymerase chain reaction (competitive PCR) for the detection and quantitation of gene expression in small tumor samples was evaluated. DNA polymerase-beta gene expression was detected in human ovarian cancer cell lines displaying a different degree of cisplatin resistance. The level of DNA polymerase-beta cDNA in the resistant cell line was threefold that of the parental sensitive line. Our results indicate that competitive PCR is a reproducible and sensitive method to detect differences in gene expression in small samples and open the possibility of using this approach to detect DNA polymerase beta cDNA in small samples from clinical tumors.
评估了竞争性聚合酶链反应（竞争性PCR）在检测和定量小肿瘤样本中基因表达方面的可靠性。在显示出不同程度顺铂耐药性的人卵巢癌细胞系中检测到了DNA聚合酶β基因表达。耐药细胞系中DNA聚合酶β cDNA的水平是亲本敏感细胞系的三倍。我们的结果表明，竞争性PCR是一种可重复且灵敏的方法，用于检测小样本中基因表达的差异，并为使用该方法检测临床肿瘤小样本中的DNA聚合酶β cDNA提供了可能性。
