Bennett S L, Cullen J B, Sherer D M, Woods J R
Department of Obstetrics and Gynecology, Strong Memorial Hospital, University of Rochester School of Medicine and Dentistry, New York 14642.
Am J Perinatol. 1993 Mar;10(2):101-4. doi: 10.1055/s-2007-994637.
Most studies investigating the usefulness of ferning and nitrazine methods for detecting the presence of amniotic fluid following ruptured amniotic membranes have employed samples from vaginal pooling. Reported accuracies are 84 to 100% for the ferning test and 87 to 97% for the nitrazine test. Our study was designed to determine if these tests are applicable throughout gestation. Samples were obtained from amniocenteses between 14 and 42 weeks. Part I: Of 112 samples allowed to dry on a slide for 3 minutes only, 86.6% were ferning positive and 100% were nitrazine positive. Flame-drying increased the presence of ferning to 96.4%. Part II: 363 samples were allowed to dry completely for up to 10 minutes. All samples were ferning and nitrazine positive. Part III: 17 samples were tested for ferning and nitrazine at 1 hour, and again at 1 week. Sixty-six samples were tested within 1 week and again at 2 weeks. All samples remained ferning and nitrazine positive. Therefore optimal analysis of suspected amniotic fluid occurs when specimens are air-dried for 10 minutes. These tests may be reliably performed at gestations of 12 to 41 weeks.
大多数研究调查羊膜破裂后使用羊齿状结晶法和石蕊试纸法检测羊水的有效性时，采用的是阴道积液样本。据报道，羊齿状结晶试验的准确率为84%至100%，石蕊试纸试验的准确率为87%至97%。我们的研究旨在确定这些测试是否适用于整个孕期。样本取自14至42周的羊膜腔穿刺术。第一部分：112个样本仅在载玻片上干燥3分钟，其中86.6%的样本羊齿状结晶呈阳性，100%的样本石蕊试纸呈阳性。火焰干燥使羊齿状结晶的出现率提高到96.4%。第二部分：363个样本完全干燥长达10分钟。所有样本的羊齿状结晶和石蕊试纸均呈阳性。第三部分：17个样本在1小时时进行羊齿状结晶和石蕊试纸检测，1周后再次检测。66个样本在1周内检测，2周后再次检测。所有样本的羊齿状结晶和石蕊试纸仍呈阳性。因此，当标本风干10分钟时，对疑似羊水进行最佳分析。这些测试在12至41周的孕期内均可可靠进行。
