Chowdhury P, Doi R, Chang L W, Rayford P L
Department of Physiology, University of Arkansas for Medical Sciences, Little Rock 72205.
Biomed Environ Sci. 1993 Mar;6(1):59-64.
This study was conducted in adult male Sprague--Dawley rats to determine the distribution of [3H]-nicotine in blood and tissues following a bolus injection and a constant infusion of pure nicotine. The animals were anesthetized and injected with either 0.5 ml of nicotine solution or given a constant infusion of the same nicotine solution with identical amounts of radioactive nicotine. After sacrifice, blood, brain, trachea, salivary gland, esophagus, lung, heart, liver, fundus, antrum, spleen, pancreas, duodenum, jejunum, ileum, cecum, colon, kidneys, adrenal gland, and testes were collected and measured for radioactivity by scintillation counting. The distribution of nicotine was found highest in kidneys by both routes of administration. Higher accumulations were also found in salivary and adrenal glands, fundus, antrum, duodenum, jejunum, ileum and colon. Retention of nicotine via constant infusion was significantly higher in esophagus, fundus antrum, spleen, cecum, pancreas, testes, heart and muscle when compared with bolus injection. Six-fold increase in retention of blood levels of nicotine were found with constant infusion. (P < 0.05). The results indicate that longer retention of nicotine occurs in blood and other specific tissues such as esophagus, fundus, antrum, spleen, cecum, pancreas, testes, heart and muscle via constant exposure. These data may implicate the predisposition of these tissues to pathologic manifestations.
本研究在成年雄性Sprague-Dawley大鼠中进行，以确定单次推注和持续输注纯尼古丁后，[3H] - 尼古丁在血液和组织中的分布情况。将动物麻醉后，要么注射0.5毫升尼古丁溶液，要么以相同量的放射性尼古丁持续输注相同的尼古丁溶液。处死后，收集血液、脑、气管、唾液腺、食管、肺、心脏、肝脏、胃底、胃窦、脾脏、胰腺、十二指肠、空肠、回肠、盲肠、结肠、肾脏、肾上腺和睾丸，并通过闪烁计数测量放射性。通过两种给药途径发现，尼古丁在肾脏中的分布最高。在唾液腺、肾上腺、胃底、胃窦、十二指肠、空肠、回肠和结肠中也发现了较高的蓄积。与单次推注相比，持续输注时，食管、胃底、胃窦、脾脏、盲肠、胰腺、睾丸、心脏和肌肉中尼古丁的滞留量显著更高。持续输注时，血液中尼古丁的滞留量增加了六倍。（P < 0.05）。结果表明，通过持续暴露，尼古丁在血液和其他特定组织如食管、胃底、胃窦、脾脏、盲肠、胰腺、睾丸、心脏和肌肉中的滞留时间更长。这些数据可能暗示这些组织易出现病理表现。
