Germain R N, Margulies D H
Lymphocyte Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Annu Rev Immunol. 1993;11:403-50. doi: 10.1146/annurev.iy.11.040193.002155.
T lymphocytes with alpha beta receptors recognize antigen in association with the polymorphic products of the class I and class II loci of the major histocompatibility complex (MHC). This presentation of antigen results from the intracellular generation of protein fragments, and the binding and transport to the cell surface of these peptides in stable association with the MHC class I and class II molecules. Each class of MHC molecule appears specialized for capture of peptides present in a particular intracellular compartment. We describe here the structural basis of peptide-MHC molecule interaction, the differences in biochemical behavior that focus the two classes of MHC molecules on peptides of distinct size and location, and the cell biology of MHC molecule transport, peptide generation, and intracellular movement. The importance of conformational changes accompanying peptide binding that affect subunit stability of MHC molecules, and the relationship between these changes and the handling of proteins by intracellular chaperones, are emphasized as key features in the operation of the class I and class II presentation pathways.
具有αβ受体的T淋巴细胞识别与主要组织相容性复合体（MHC）I类和II类基因座的多态性产物相关的抗原。这种抗原呈递源于细胞内蛋白质片段的产生，以及这些肽与MHC I类和II类分子稳定结合并转运至细胞表面。每一类MHC分子似乎专门用于捕获特定细胞内区室中存在的肽。我们在此描述肽-MHC分子相互作用的结构基础、使两类MHC分子聚焦于不同大小和位置的肽的生化行为差异，以及MHC分子转运、肽产生和细胞内移动的细胞生物学。强调了伴随肽结合的构象变化对MHC分子亚基稳定性的影响，以及这些变化与细胞内伴侣蛋白对蛋白质处理之间的关系，它们是I类和II类呈递途径运作中的关键特征。
