Forty J, White D J, Wallwork J
Papworth Hospital, Cambridge, UK.
J Heart Lung Transplant. 1993 Mar-Apr;12(2):283-6; discussion 286-7.
Isolated rabbit hearts were perfused as autoperfusing working preparations with human blood to simulate discordant hyperacute xenograft rejection. Perfusion with unmodified human blood resulted in immediate thrombotic failure of the hearts. This process was initiated by immunoglobulin M heterophile human anti-rabbit antibody, mediated by the classic pathway of complement and platelet-activating factor, and effected by platelets. Prevention of this process resulted in organ damage by another process occurring at about 20 minutes. Removal of heterophile antibody from the perfusing blood had no effect on this second event, but specific inactivation of the alternative pathway of complement prevented its generation despite normal titers of antibody and normal classic pathway activity. Studies of the deposition of components of the human complement system on the endothelium of the xenoperfused hearts confirm a role for the alternative pathway of complement in xenograft rejection in this discordant species combination.
将离体兔心作为自身灌注的工作标本，用人血灌注以模拟不协调性超急性异种移植排斥反应。用未修饰的人血灌注会导致心脏立即发生血栓性衰竭。这个过程由人抗兔异嗜性免疫球蛋白M抗体引发，由补体经典途径和血小板活化因子介导，并由血小板起作用。防止这个过程会导致大约20分钟时发生的另一个过程造成器官损伤。从灌注血液中去除异嗜性抗体对这第二个事件没有影响，但补体替代途径的特异性失活可防止其发生，尽管抗体滴度正常且经典途径活性正常。对人补体系统成分在异种灌注心脏内皮上沉积的研究证实了补体替代途径在这种不协调性物种组合的异种移植排斥反应中的作用。
