Weihe E, Nohr D, Sharer L, Murray E, Rausch D, Eiden L
Department of Anatomy, University of Mainz, Germany.
Neuroreport. 1993 Mar;4(3):263-6. doi: 10.1097/00001756-199303000-00009.
The pattern of expression of GFAP immunoreactivity in astrocytes of the juvenile rhesus monkey cortex was examined following infection with simian immunodeficiency virus (SIV). Blocks of cerebral cortex plus subjacent white matter from saline- and formalin-perfused brain were examined by peroxidase-linked immunochemical and immunofluorescence staining of deparaffinized sections. Strong GFAP immunoreactivity was found in astrocytic cells in both uninfected and SIV-infected juvenile macaque in the subpial cerebral cortex and in subcortical white matter, where GFAP-positive cells were abundant. GFAP staining of cortical layers 2-6 on the other hand was weak or absent in three uninfected controls and one infected animal without cognitive impairment, but moderate to strong in animals productively infected with SIV that demonstrated cognitive and/or motor impairment. These data demonstrate a cortical locus of astrocytic activation in rhesus monkeys infected with primate immunodeficiency virus isolate SIVB670 which, like HIV-1 in man, causes motor/cognitive impairment as well as immunodeficiency disease.
在感染猿猴免疫缺陷病毒（SIV）后，对幼年恒河猴皮层星形胶质细胞中胶质纤维酸性蛋白（GFAP）免疫反应性的表达模式进行了检测。通过对脱石蜡切片进行过氧化物酶联免疫化学和免疫荧光染色，检查了来自生理盐水灌注和福尔马林灌注大脑的大脑皮层及下方白质块。在未感染和感染SIV的幼年猕猴的软脑膜下大脑皮层和皮层下白质中的星形胶质细胞中发现了强烈的GFAP免疫反应性，其中GFAP阳性细胞丰富。另一方面，在三只未感染对照动物和一只无认知障碍的感染动物中，皮层第2 - 6层的GFAP染色较弱或无染色，但在有认知和/或运动障碍的SIV有效感染动物中，GFAP染色为中度至强阳性。这些数据表明，感染灵长类免疫缺陷病毒分离株SIVB670的恒河猴存在星形胶质细胞激活的皮层位点，该病毒与人类的HIV-1一样，会导致运动/认知障碍以及免疫缺陷疾病。
