O'Hearn E, Long D B, Molliver M E
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Neuroreport. 1993 Mar;4(3):299-302. doi: 10.1097/00001756-199303000-00018.
Ibogaine, an indole alkaloid, has been proposed for treatment of drug addiction, yet its mechanism, site of action, and possible neurotoxicity have not been determined. Since neuronal injury is known to activate neurologlial cells, we investigated potential neurotoxic effects of this drug in rats by examining expression of specific glial markers. After treatment with ibogaine (100 mg kg-1 i.p.; 1-3 doses), we observed increased cytochemical markers in both microglia (OX-6, OX-42, W3/25) and astrocytes (GFAP), associated with striking morphologic changes in these cells. Activated glial cells were restricted to longitudinally oriented, parasagittal stripes within the vermis of cerebellar cortex. The ibogaine-induced activation of cerebellar glial cells is highly suggestive of neuronal degeneration, most likely of Purkinje cells.
伊博格碱是一种吲哚生物碱，已被提议用于治疗药物成瘾，但其作用机制、作用部位及可能的神经毒性尚未明确。由于已知神经元损伤会激活神经胶质细胞，我们通过检测特定胶质细胞标志物的表达，研究了该药物对大鼠潜在的神经毒性作用。在用伊博格碱（100 mg/kg腹腔注射；1 - 3剂）治疗后，我们观察到小胶质细胞（OX - 6、OX - 42、W3/25）和星形胶质细胞（GFAP）中的细胞化学标志物均增加，且这些细胞出现了显著的形态学变化。活化的神经胶质细胞局限于小脑皮质蚓部纵向排列的矢状旁条纹内。伊博格碱诱导的小脑神经胶质细胞活化强烈提示神经元变性，最有可能是浦肯野细胞变性。
