Ramakrishna V, Eisenthal A, Skornick Y, Shinitzky M
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Cancer Immunol Immunother. 1993 May;36(5):293-9. doi: 10.1007/BF01741167.
The B16-BL6 melanoma, like most spontaneously arising tumors, is poorly immunogenic and expresses low levels of major histocompatibility complex (MHC) antigens. Treatment of cells of this tumor in vitro by hydrostatic pressure in the presence of adenosine 2',3'-dialdehyde (oxAdo), a membrane-impermeant crosslinker, caused elevated projection of MHC and a specific tumor antigen as demonstrated by flow-cytometric analysis. Maximum projection of both the MHC and the tumor antigens could be reached by application of 1200 atm for 15 min in the presence of 20 mM oxAdo. It is not yet clear whether this passive increase in availability of antigens on the cell surface originated from a dormant pool of antigens in the plasma membrane or from pressure-induced fusion of antigen-rich intracellular organelles (e.g. the endoplasmic reticulum). The immunogenic properties of the antigen-enriched B16-BL6 cells are described in the following paper.
与大多数自发产生的肿瘤一样,B16 - BL6黑色素瘤免疫原性较差,主要组织相容性复合体(MHC)抗原表达水平较低。在膜不通透性交联剂腺苷2',3'-二醛(oxAdo)存在的情况下,通过流体静压在体外处理该肿瘤细胞,流式细胞术分析表明,MHC和一种特异性肿瘤抗原的表达增加。在20 mM oxAdo存在的情况下,施加1200个大气压15分钟可使MHC和肿瘤抗原的表达达到最大值。目前尚不清楚细胞表面抗原可用性的这种被动增加是源于质膜中休眠的抗原池,还是源于压力诱导的富含抗原的细胞内细胞器(如内质网)的融合。富集抗原的B16 - BL6细胞的免疫原性特性在以下论文中进行了描述。