Goldberg D, Marbach I, Gross E, Levitzki A, Simchen G
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Eur J Biochem. 1993 Apr 1;213(1):195-204. doi: 10.1111/j.1432-1033.1993.tb17748.x.
We have cloned, by functional complementation of the cdc25-2 mutation of Saccharomyces cerevisiae, a homolog of CDC25 from the pathogenic yeast Candida albicans. The new gene, named CSC25 codes for a 1333-amino-acid protein. The full length gene, as well as a truncated form coding for 795 amino acids, suppresses the thermosensitive phenotype of cdc25ts mutants. Biochemical analysis has shown that Csc25 activates the Ras/adenylyl cyclase pathway in S. cerevisiae at a rate two to three times faster than Cdc25, under the same conditions. The C-terminal domain of Csc25 is highly similar to the C-terminal domain of Cdc25, to almost the same extent as the C-terminus of the endogenous Cdc25 homolog Sdc25. We show that polyclonal anti-Cdc25 antibodies interact with Csc25 expressed in S. cerevisiae. In addition to the full length protein (approximately 150 kDa), we have found a approximately 50-kDa polypeptide which seems to include the C-terminus of the CSC25 gene product.
我们通过对酿酒酵母cdc25 - 2突变体进行功能互补，克隆出了致病酵母白色念珠菌中CDC25的一个同源基因。这个名为CSC25的新基因编码一个1333个氨基酸的蛋白质。全长基因以及编码795个氨基酸的截短形式，都能抑制cdc25ts突变体的温度敏感表型。生化分析表明，在相同条件下，Csc25激活酿酒酵母中Ras/腺苷酸环化酶途径的速度比Cdc25快两到三倍。Csc25的C末端结构域与Cdc25的C末端结构域高度相似，其相似程度几乎与内源性Cdc25同源物Sdc25的C末端相同。我们发现多克隆抗Cdc25抗体能与酿酒酵母中表达的Csc25相互作用。除了全长蛋白（约150 kDa）外，我们还发现了一个约50 kDa的多肽，它似乎包含CSC25基因产物的C末端。
