Kimura A, Nakashima S, Uda T, Ikeda H, Yasuda S, Tsuji T, Matsumura S
Department of Legal Medicine, Wakayama Medical College, Japan.
Eur J Biochem. 1993 Apr 1;213(1):5-10. doi: 10.1111/j.1432-1033.1993.tb17728.x.
The heavy-chain isoforms of myosin in human non-muscle and smooth muscle tissues were analyzed by means of SDS/PAGE and using three distinct newly developed monoclonal anti-(human cerebrum myosin) Ig (HBM-1, HBM-3 and HBM-4). Purified cerebrum myosin contained three electrophoretic variants of non-muscle myosin heavy chain (NM1, NM2 and NM3, with apparent molecular masses of about 200, 198 and 196 kDa, respectively). Both NM1 and NM2 were recognizable by the brain-specific antibody HBM-1, while NM3 was recognizable by HBM-3. Each of the variants reacted with HBM-4 to a similar extent. Purified cerebellum myosin gave three electrophoretic variants of the heavy chain which were indistinguishable electrophoretically or immunologically from those of cerebrum myosin. Aortic myosin contained four electrophoretic variants, including the two smooth muscle myosin heavy chain isoforms and NM2-like and NM3-like heavy chains. Liver, platelet and kidney myosins contained a heavy chain very similar to NM3. Kidney myosin also contained a small fraction of an NM2-like electrophoretic variant. In addition, cerebrum, kidney, liver and platelet myosins appeared to contain minor, 194-kDa myosin heavy-chain-like polypeptide(s) (NM4). NM1, as well as NM2 and NM3, thus appear to be the brain-type and non-brain-type non-muscle myosin heavy-chain isoforms, respectively, and additional minor heavy-chain isoforms are also likely to be present in human tissues.
利用SDS/PAGE并使用三种新开发的不同的单克隆抗（人脑肌球蛋白）Ig（HBM-1、HBM-3和HBM-4），对人非肌肉和平滑肌组织中的肌球蛋白重链异构体进行了分析。纯化的脑肌球蛋白包含非肌肉肌球蛋白重链的三种电泳变体（NM1、NM2和NM3，表观分子量分别约为200、198和196 kDa）。NM1和NM2均可被脑特异性抗体HBM-1识别，而NM3可被HBM-3识别。每种变体与HBM-4的反应程度相似。纯化的小脑肌球蛋白产生了重链的三种电泳变体，其在电泳或免疫方面与脑肌球蛋白的变体无法区分。主动脉肌球蛋白包含四种电泳变体，包括两种平滑肌肌球蛋白重链异构体以及NM2样和NM3样重链。肝脏、血小板和肾脏肌球蛋白包含一条与NM3非常相似的重链。肾脏肌球蛋白还包含一小部分NM2样电泳变体。此外，脑、肾脏、肝脏和血小板肌球蛋白似乎包含少量194 kDa的肌球蛋白重链样多肽（NM4）。因此，NM1以及NM2和NM3似乎分别是脑型和非脑型非肌肉肌球蛋白重链异构体，并且人组织中也可能存在其他少量的重链异构体。
