Sierra A, Rubio N
Instituto Cajal, Department of Neuroimmunology, Madrid, Spain.
Immunology. 1993 Mar;78(3):399-404.
Cytokines have been postulated to exert an important modulatory and recruiting role in demyelination induced by Theiler's murine encephalomyelitis virus (TMEV) in SJL/J mice. Using a cytolytic bioassay and ELISA, we have detected and quantified a cytokine, tumour necrosis factor-alpha (TNF-alpha), in supernatants from astrocyte cultures infected in vitro with TMEV. TNF was detected only after TMEV-specific infection of astrocyte cultures (approximately 200-400 U/ml). In vitro TNF synthesis appeared in a dose- and time-dependent manner and was produced by both SJL/J (a strain susceptible to TMEV-induced demyelination) and BALB/c (a resistant strain) astrocytes. The precise nature of TNF activity was further assessed by fast protein liquid chromatography (FPLC) and antibody neutralization. These results indicate an active role for astrocytes as accessory immune cells in our experimental model for multiple sclerosis.
细胞因子被认为在SJL/J小鼠中由泰勒氏鼠脑脊髓炎病毒（TMEV）诱导的脱髓鞘过程中发挥重要的调节和募集作用。我们使用细胞溶解生物测定法和酶联免疫吸附测定法（ELISA），检测并定量了体外感染TMEV的星形胶质细胞培养上清液中的一种细胞因子——肿瘤坏死因子-α（TNF-α）。TNF仅在星形胶质细胞培养物被TMEV特异性感染后才被检测到（约200 - 400 U/ml）。体外TNF合成呈剂量和时间依赖性，且由SJL/J（对TMEV诱导的脱髓鞘敏感的品系）和BALB/c（抗性品系）星形胶质细胞产生。通过快速蛋白质液相色谱（FPLC）和抗体中和进一步评估了TNF活性的确切性质。这些结果表明，在我们的多发性硬化症实验模型中，星形胶质细胞作为辅助免疫细胞发挥着积极作用。
