MacDonald H R, Casanova J L, Maryanski J L, Cerottini J C
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
J Exp Med. 1993 May 1;177(5):1487-92. doi: 10.1084/jem.177.5.1487.
Previous T cell receptor (TCR) sequence analysis of a panel of 23 H-2Kd restricted cytotoxic T lymphocyte (CTL) clones recognizing the decapeptide HLA-CW3 170-179 revealed a striking conservation of TCR structure, in that all clones examined used V beta 10 and J alpha pHDS58 segments. We show here that the primary response in vivo after intraperitoneal injection of DBA/2 mice with HLA-CW3 expressing transfectants of syngeneic P815 (H-2d) tumor cells is characterized by a dramatic expansion of CD8+ V beta 10+ CTL in the peritoneal cavity and draining (mesenteric) lymph node, as well as in peripheral blood. Additional analysis of TCR on HLA-CW3 immune populations by flow cytometry and polymerase chain reaction further indicates that the vast majority of responding CD8+ cells express restricted V alpha domains, a dominant J alpha segment (pHDS58), and a conserved CDR3 length for both alpha and beta chains. This novel system provides a unique opportunity to directly monitor an oligoclonal primary antigen specific immune response in vivo at the single cell level independently of functional assays.
先前对一组23个识别十肽HLA - CW3 170 - 179的H - 2Kd限制性细胞毒性T淋巴细胞（CTL）克隆进行的T细胞受体（TCR）序列分析显示，TCR结构存在显著保守性，即所有检测的克隆都使用Vβ10和JαpHDS58片段。我们在此表明，向DBA / 2小鼠腹腔注射表达HLA - CW3的同基因P815（H - 2d）肿瘤细胞转染体后，体内的初始反应特征是腹腔、引流（肠系膜）淋巴结以及外周血中CD8 + Vβ10 + CTL显著扩增。通过流式细胞术和聚合酶链反应对HLA - CW3免疫群体上的TCR进行的进一步分析表明，绝大多数反应性CD8 +细胞表达受限的Vα结构域、一个占主导地位的Jα片段（pHDS58），并且α链和β链的互补决定区3（CDR3）长度保守。这个新系统提供了一个独特的机会，可在单细胞水平直接监测体内寡克隆性初始抗原特异性免疫反应，而无需依赖功能测定。
