Sheppard C J, Gu M
Physical Optics Department, School of Physics, University of Sydney, N.S.W., Australia.
J Microsc. 1993 Mar;169(Pt 3):339-45. doi: 10.1111/j.1365-2818.1993.tb03310.x.
Striated muscle fibres can be modelled by a simple geometry, which has allowed three-dimensional (3-D) images in conventional and confocal microscopes to be calculated. This model is useful for comparing different imaging methods and represents a simple example of an application of the 3-D optical transfer function (OTF) for the system. The rejection of out-of-focus blur is demonstrated, and the effects of fibre thickness and confocal pinhole size on image contrast are investigated. The effects of using a simple filter for image enhancement are studied, elucidating the characteristics of the OTF.
横纹肌纤维可以用一种简单的几何形状来建模，这使得传统显微镜和共聚焦显微镜中的三维（3-D）图像得以计算。该模型有助于比较不同的成像方法，并且代表了三维光学传递函数（OTF）在该系统中应用的一个简单示例。展示了对离焦模糊的抑制，并研究了纤维厚度和共聚焦针孔大小对图像对比度的影响。研究了使用简单滤波器进行图像增强的效果，阐明了OTF的特性。
