Stover P, Kruschwitz H, Schirch V, Wright H T
Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298-0614.
J Mol Biol. 1993 Apr 5;230(3):1094-6. doi: 10.1006/jmbi.1993.1225.
Diffraction grade crystals of serine hydroxymethyltransferase were obtained after extensive screening of source of enzyme, purification procedures, ligand binding and crystallization conditions. Serine hydroxymethyltransferase purified from Escherichia coli crystallizes in two forms that are suitable for crystal structure determination.
在对酶的来源、纯化程序、配体结合和结晶条件进行广泛筛选后，获得了丝氨酸羟甲基转移酶的衍射级晶体。从大肠杆菌中纯化的丝氨酸羟甲基转移酶以两种适合晶体结构测定的形式结晶。
