Cooper K F, Fisher R B, Tyler-Smith C
Department of Biochemistry, University of Oxford, U.K.
J Mol Biol. 1993 Apr 5;230(3):787-99. doi: 10.1006/jmbi.1993.1201.
Eighteen yeast artificial chromosome (YAC) clones containing alphoid satellite DNA and adjacent sequences from the human Y chromosome have been identified from three different YAC libraries. Restriction site mapping of the genomic alphoid arrays and the YACs has allowed seven of the alphoid clones to be positioned on the arrays. Three clones extend into flanking sequences. At one edge the alphoid DNA is highly diverged and is flanked by a small block of the 48 base-pair satellite, dispersed moderately repeated sequences and a separate short alphoid array. More distal sequences are Y-specific. At the other edge there is much less divergence and the alphoid DNA is flanked by an Alu sequence and the five base-pair satellite.
从三个不同的酵母人工染色体（YAC）文库中鉴定出了18个含有α卫星DNA及来自人类Y染色体相邻序列的YAC克隆。对基因组α卫星阵列和YAC进行限制酶切位点图谱分析，已将7个α卫星克隆定位到这些阵列上。三个克隆延伸至侧翼序列。在一侧边缘，α卫星DNA高度分化，侧翼是一小段48碱基对的卫星序列、中度分散的重复序列和一个单独的短α卫星阵列。更远端的序列是Y染色体特异性的。在另一侧边缘，分化程度低得多，α卫星DNA侧翼是一个Alu序列和5碱基对的卫星序列。
