Egan S E, Giddings B W, Brooks M W, Buday L, Sizeland A M, Weinberg R A
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Nature. 1993 May 6;363(6424):45-51. doi: 10.1038/363045a0.
The proteins Grb2-Sem-5, Shc and Sos have been implicated in the signalling pathway from tyrosine kinase receptors to Ras. Grb2-Sem-5 binds directly to murine Sos1, a Ras exchange factor, through two SH3 domains. Sos is also associated with ligand-activated tyrosine kinase receptors which bind Grb2-Sem-5, and with the Grb2-Sem-5 binding protein, Shc. Ectopic expression of Drosophila Sos stimulates morphological transformation of rodent fibroblasts. These data define a pathway by which tyrosine kinases act through Ras to control cell growth and differentiation.
蛋白质Grb2-Sem-5、Shc和Sos参与了从酪氨酸激酶受体到Ras的信号通路。Grb2-Sem-5通过两个SH3结构域直接与鼠源Sos1（一种Ras交换因子）结合。Sos还与结合Grb2-Sem-5的配体激活型酪氨酸激酶受体以及Grb2-Sem-5结合蛋白Shc相关联。果蝇Sos的异位表达可刺激啮齿动物成纤维细胞的形态转化。这些数据定义了一条酪氨酸激酶通过Ras作用来控制细胞生长和分化的途径。
