1,25-二羟基维生素D3和己酮可可碱（曲克芦丁）诱导急性早幼粒细胞白血病细胞分化
Differentiation of acute promyelocytic leukemia cell induced by 1,25-dihydroxyvitamin D3 and pentoxifylline (Trental).
作者信息
Srivastava M, Ambrus J L
机构信息
State University of New York, Buffalo Graduate School, Roswell Park Division, Department of Experimental Pathology, NY.
出版信息
Res Commun Chem Pathol Pharmacol. 1993 Mar;79(3):385-8.
Human promyelocytic leukemia cell line HL-60 in tissue culture was treated with various concentrations of 1,25-dihydroxyvitamin D3, (D3) pentoxifylline (Px) or in combinations. Differentiation to normal granulocytes or monocytes was evaluated by nitroblue tetrazolium (NBT) reducing capacity and morphologic criteria. (D3) produced monocytic differentiation, but sub-differentiation inducing doses of (Px) had no effect. In previous studies we found higher doses of (Px) to produce granulocytic differentiation in vitro and in vivo.
将组织培养中的人早幼粒细胞白血病细胞系HL - 60用不同浓度的1,25 - 二羟维生素D3、己酮可可碱（Px）或其组合进行处理。通过硝基蓝四氮唑（NBT）还原能力和形态学标准评估向正常粒细胞或单核细胞的分化情况。1,25 - 二羟维生素D3诱导单核细胞分化，但己酮可可碱的亚分化诱导剂量无效。在先前的研究中，我们发现更高剂量的己酮可可碱在体外和体内均可诱导粒细胞分化。
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