Matsumoto H
Division of Food Hygiene, Osaka Prefectural Institute of Public Health, Japan.
Arch Toxicol. 1993;67(2):98-103. doi: 10.1007/BF01973678.
The induction by airborne particulate extracts of aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin O-deethylase (ECOD) in early chick embryos was studied to determine whether or not lethality by the extracts was related to the induction. The optimal pH values for AHH and ECOD were 7.1 and 6.9, respectively, after administering the extracts to early embryos. The AHH inducing capacity was significantly increased 4 h after injection of the extracts, then the value gradually increased. The ECOD inducing ability was similar to that of AHH, although to a lesser extent. Among fractions from the extracts, the neutral fraction was responsible for most of the AHH inducing activity. Two neutral subfractions (N2 and N3), consisting mostly of polycyclic aromatic hydrocarbons (PAH) and their derivatives, had the highest inducing activity. A dose-response relationship was observed between the amounts of the N2 and induction of AHH and ECOD in 8-day-old embryos. When the N2 was administered at different stages of development (days 5-9), the values of induced AHH and ECOD activities increased along with the days of development. Extracts of the smaller airborne particulates (< 1.1 and 1.1-2.0 microns cut-off diameters) had stronger AHH and ECOD inducing abilities than those of the larger, since the former contained high levels of PAH and their derivatives. These results suggested that PAH and their derivatives in the airborne particulates induced AHH and ECOD activities in the early chick embryo. The relationship between the extent of AHH and ECOD inducing activities and that of embryolethality caused by extracts of airborne particulates was not well established.
研究了空气传播颗粒提取物对早期鸡胚中芳烃羟化酶（AHH）和7-乙氧基香豆素O-脱乙基酶（ECOD）的诱导作用，以确定提取物的致死性是否与诱导作用相关。将提取物施用于早期胚胎后，AHH和ECOD的最佳pH值分别为7.1和6.9。注射提取物后4小时，AHH诱导能力显著增加，然后该值逐渐升高。ECOD诱导能力与AHH相似，尽管程度较小。在提取物的各组分中，中性组分负责大部分AHH诱导活性。两个主要由多环芳烃（PAH）及其衍生物组成的中性亚组分（N2和N3）具有最高的诱导活性。在8日龄胚胎中，观察到N2的量与AHH和ECOD诱导之间的剂量反应关系。当在不同发育阶段（第5 - 9天）施用N2时，诱导的AHH和ECOD活性值随发育天数增加。空气传播的较小颗粒（截止直径<1.1和1.1 - 2.0微米）的提取物比大颗粒提取物具有更强的AHH和ECOD诱导能力，因为前者含有高水平的PAH及其衍生物。这些结果表明，空气传播颗粒中的PAH及其衍生物诱导早期鸡胚中的AHH和ECOD活性。空气传播颗粒提取物引起的AHH和ECOD诱导活性程度与胚胎致死率之间的关系尚未明确确立。
