Akin C, Sonnenfeld G
Department of Microbiology and Immunology, University of Louisville, KY 40292.
Cancer Lett. 1993 Apr 15;69(1):51-7. doi: 10.1016/0304-3835(93)90032-5.
The effects of insulin and granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of transferrin receptors in AML-193 myeloid leukemia cell line were investigated. Insulin, alone or in combination with GM-CSF, caused an increase in surface transferrin receptors within 5 min. This increase was inhibited by hyperosmolarity but not by cycloheximide suggesting the involvement of receptor recycling from internal pools. In contrast, receptor increase after 18 h of incubation with insulin and GM-CSF was sensitive to cycloheximide indicating that long term effects of these growth factors are mediated through protein synthesis. These results may have implications on combined GM-CSF + antitransferrin receptor antibody therapy of leukemias.
研究了胰岛素和粒细胞巨噬细胞集落刺激因子（GM-CSF）对AML-193髓系白血病细胞系中转铁蛋白受体表达的影响。单独使用胰岛素或与GM-CSF联合使用，均可在5分钟内使表面转铁蛋白受体增加。这种增加被高渗抑制，但不被环己酰亚胺抑制，提示受体从内部池循环参与其中。相反，与胰岛素和GM-CSF孵育18小时后受体增加对环己酰亚胺敏感，表明这些生长因子的长期作用是通过蛋白质合成介导的。这些结果可能对白血病的GM-CSF +抗转铁蛋白受体抗体联合治疗有影响。
