借助碱性磷酸酶进行尿苷二磷酸葡萄糖醛酸焦磷酸酶测定。
UDPglucuronic acid pyrophosphatase assay with the aid of alkaline phosphatase.
作者信息
Puhakainen E, Saarinen A, Hänninen O
出版信息
Acta Chem Scand B. 1977 Mar;B31(2):125-9. doi: 10.3891/acta.chem.scand.31b-0125.
A simple and sensitive method has been described for the determination of UDPglucuronic acid pyrophosphatase activity. Pyrophosphatase-free alkaline phosphatase preparation is added to the reaction mixture in order to hydrolyze the phosphate esters (UMP and alpha-D-glucuronic acid 1-phosphate) produced by pyrophosphatase. The inorganic phosphate liberated is measured by a modification of Fiske and SubbaRow's method. The phosphatase coupled method is time saving, easy to perform and accurate. It can also be used for pyrophosphatase assays with other nucleotide substrates like UDPglucose, UDP-N-acetylglucosamine, NAD+, NADH, NADP+ and NADPH.
已描述了一种用于测定UDP葡萄糖醛酸焦磷酸酶活性的简单且灵敏的方法。将不含焦磷酸酶的碱性磷酸酶制剂加入反应混合物中，以水解焦磷酸酶产生的磷酸酯（UMP和α-D-葡萄糖醛酸1-磷酸）。通过对Fiske和SubbaRow方法的改进来测量释放的无机磷酸盐。磷酸酶偶联法省时、易于操作且准确。它还可用于用其他核苷酸底物（如UDP葡萄糖、UDP-N-乙酰葡糖胺、NAD+、NADH、NADP+和NADPH）进行的焦磷酸酶测定。
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