3-氟-4-去甲氧基柔红霉素与六核苷酸d(TCCGGA)2相互作用的19F核磁共振研究。

A 19F NMR study of the interaction of 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2.

作者信息

Barr K E, Russell R A, Warrener R N, Collins J G

机构信息

Department of Chemistry, University College, University of New South Wales, Australia.

出版信息

FEBS Lett. 1993 May 10;322(2):173-6. doi: 10.1016/0014-5793(93)81562-e.

DOI:10.1016/0014-5793(93)81562-e

PMID:8482387

Abstract

19F NMR spectroscopy has been used to study the binding of the fluorinated anthracycline 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2. In the spectrum of the 1:1 anthracycline-d(TCCGGA)2 complex four resonances of approximately equal intensity were observed. This indicated that 4-demethoxydaunomycin intercalated at all possible sites with similar affinity. This suggests that the specific high affinity binding sites that are observed in anthracycline-DNA footprinting experiments are strongly regulated by the local DNA conformation.

摘要

19F核磁共振光谱已被用于研究氟化蒽环类药物3-氟-4-去甲氧基柔红霉素与六核苷酸d(TCCGGA)2的结合。在1:1蒽环类药物-d(TCCGGA)2复合物的光谱中，观察到四个强度近似相等的共振峰。这表明4-去甲氧基柔红霉素以相似的亲和力插入到所有可能的位点。这表明在蒽环类药物-DNA足迹实验中观察到的特定高亲和力结合位点受到局部DNA构象的强烈调控。