Doronin K K, Zakharchuk A N, Grinenko N F, Yurov G K, Krougliak V A, Naroditsky B S
Institute of Agricultural Biotechnology, Moscow, Russian Federation.
Gene. 1993 Apr 30;126(2):247-50. doi: 10.1016/0378-1119(93)90374-c.
A nondefective recombinant human adenovirus 5 (Ad5) carrying the SEAP gene, encoding human secreted placental alkaline phosphatase, in the E3 region of the Ad5 genome was obtained. The expression of SEAP at the early and late stages of Ad5 infection was demonstrated in permissive and semi-permissive cell cultures. The amount of SEAP in the culture medium of the 293 cells was 13.6% of the total protein.
获得了一种携带SEAP基因的无缺陷重组人腺病毒5（Ad5），该基因编码人分泌型胎盘碱性磷酸酶，位于Ad5基因组的E3区域。在允许性和半允许性细胞培养中证实了Ad5感染早期和晚期SEAP的表达。293细胞培养基中SEAP的量占总蛋白的13.6%。
