Sugita M, Tenjin Y
Dept. of Gynecology, Sasaki Institute Kyoundo Hospital.
Nihon Rinsho. 1993 Apr;51(4):1108-13.
Confocal laser scanning microscopy (CLSM) is expected to provide new optical information different from conventional microscopy in cell biology. CLSM provides high contrast images, individual continuous cellular tomograms without slicing cells, and stereographs which are observed by three dimensional reconstruction. We study the localization of nuclear DNA stained with PI, tumor marker antigen CA-125 stained with FITC, and signals in fluorescence in situ hybridization (FISH) with alpha satellite pericentrometric DNA probe of chromosome 17 to discuss the possibility of its application to cell biology or clinical use. Some of the applications of CLSM including its structure and principle are reviewed.
共聚焦激光扫描显微镜（CLSM）有望在细胞生物学领域提供与传统显微镜不同的新光学信息。CLSM能提供高对比度图像、无需对细胞进行切片的单个连续细胞断层扫描图，以及通过三维重建观察到的立体图像。我们研究了用碘化丙啶（PI）染色的核DNA、用异硫氰酸荧光素（FITC）染色的肿瘤标志物抗原CA - 125以及用17号染色体α卫星着丝粒周围DNA探针进行荧光原位杂交（FISH）中的信号定位，以探讨其应用于细胞生物学或临床用途的可能性。本文综述了CLSM的一些应用，包括其结构和原理。
