Erickson A C, Johnson G V
Department of Psychiatry and Behavioral Neurobiology, University of Alabama, Birmingham 35294-0017.
J Neurosci Methods. 1993 Mar;46(3):245-9. doi: 10.1016/0165-0270(93)90073-z.
Tau is a neuronal cytoskeletal protein consisting of a group of isoforms with apparent molecular masses ranging from 45 to 62 kDa. Tau purified from brain exists in multiple phosphorylated forms and abnormally phosphorylated tau appears to play an important role in the neuropathology of Alzheimer's disease. To separate the differentially phosphorylated populations of tau, a chromatographic technique using ferric ions adsorbed onto iminodiacetic acid substituted Sepharose was developed. Several distinct populations of tau were isolated based on the phosphorylation state. These preparations can be used for further investigation of how each specific phosphorylation state modulates the metabolism and function of tau.
tau蛋白是一种神经元细胞骨架蛋白，由一组表观分子量在45至62 kDa之间的异构体组成。从大脑中纯化的tau蛋白以多种磷酸化形式存在，异常磷酸化的tau蛋白似乎在阿尔茨海默病的神经病理学中起重要作用。为了分离tau蛋白的差异磷酸化群体，开发了一种使用吸附在亚氨基二乙酸取代的琼脂糖上的铁离子的色谱技术。根据磷酸化状态分离出了几个不同的tau蛋白群体。这些制剂可用于进一步研究每种特定的磷酸化状态如何调节tau蛋白的代谢和功能。
